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蛋白质是角膜基质的主要组成成分,由角膜上皮细胞、基质细胞、内皮细胞分泌到细胞外基质.SDS-聚丙烯酰胺凝胶分离技术和光谱测定技术已确定了在蛋白质序列数据库中标注的1 679种角膜基质蛋白质.基质蛋白在角膜的病理生理过程中具有重要的作用,对其功能的研究有助于揭示多种眼科疾病的发病机制,并为相关病变的防治提供新的思路.从基质蛋白的表现形式上看,其构成了角膜基质的凝胶样成分和纤维网架样结构,凝胶样基质包括蛋白聚糖、酶类、血清蛋白和结合蛋白,纤维网架包括胶原、弹性蛋白、角蛋白、波形蛋白以及起黏着作用的纤维连接蛋白和层黏连蛋白.本文就基质蛋白在组成和功能上的研究进展进行综述.“,”Cornea stroma mostly consists of proteins in extracellular matrix secreted by corneal epithelial cells,stroma and endothelial cells.A total of 1 679 unique Swiss-Prot annotated proteins are identified by LC-MS/MS analyses in human corneal stroma based on SDS-PAGE separation followed by in-gel trypsin digestion.As the principal component of corneal stroma, corneal stroma proteins play an important role in the physiological and pathological activities of eye.Studies towards their functions are helpful for revealing the pathogenesis of eye diseases and providing new ideas of prevention and therapy.Mainly through their manifestations,proteins in corneal stroma play a key role in forming gel-like organic material and network like structure of filaments.The gel-like organic material is composed of proteoglycan,enzymes,haemocyanin and binding proteins,while the network of filaments are constituted by collagen, elastin,keratin,vimentin as well as interconnected filaments made from fibronectin and laminin.The categories and functions of corneal stroma proteins were summarized.