葛根素抗心肌细胞缺氧复氧损伤保护作用机制研究

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目的:葛根素是一种葛根豆科植物中提取的黄酮苷类成分,研究表明:葛根素具有抗心肌缺氧复氧损伤的保护作用,属于药理学的研究热点。蛋白激酶Cε(protein kinase Cε, PKCε)属于依赖磷脂的丝/苏氨酸激酶家族,其各种亚型参预了细胞凋亡等多种细胞内病理生理功能的调控,是重要的内源性心肌保护因子之一。本实验采用了新生Sprague-Dawley(SD)大鼠原代心肌细胞,成功地建立缺氧/复氧(A/R)损伤模型,探讨PKCε蛋白表达与葛根素抗心肌细胞缺氧复氧损伤的保护作用之间的关系。方法:
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