【摘 要】
：
The identification of interaction between drugs and target proteins is a key area in drug discovery [1].The adrenergic receptor (AR) is a member of G-protein-coupled receptors (GPCRs), which could be
【机 构】
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Research Institute of Tinghua University in Shenzhen, Shenzhen 518000, China;Northwest University, X
【出 处】
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第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
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The identification of interaction between drugs and target proteins is a key area in drug discovery [1].The adrenergic receptor (AR) is a member of G-protein-coupled receptors (GPCRs), which could be divided into two subtypes: α-AR and β-AR.According to the analysis, GPCRs constitute 45% of all drug targets[2] . Organism is a complex system, and it is necessary to construct a model with multi-targets for drug discovering and designing.In our previous works, immobilized αl and β2-AR stationary phases were obtained.In this study, we constructed mixed stationary phase, which immobilized both αl-AR and β2-AR on the same silica gel, and then the stationary phase was packed into a stainless steel column to construct the mixed ARs affinity chromatography.This work was designed to reveal the binding interaction of propranolol (an agonist of β2-AR) with immobilized ARs by determining its association constants and binding sites through frontal analysis.
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