The inhibitory effects of the DNA vaccine co-expressing CEA tandem repeat epi-topes and IL-18on the

来源 :2016中华医学会呼吸病学年会暨第十七次全国呼吸病学学术会议 | 被引量 : 0次 | 上传用户:asijhvherjknvn
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  目的 To observe inhibitory effects of DNA vaccine co-expressing CEA tandem repeat epitopes and IL-18 on cancer cells in mice.方法 Methods:The encoding sequences for CEA tandem repeats and IL-18 were inserted into plasmid pcDNA3.0,constructingpcDNA-CEA625-667,pcDNA-triCEA625-667 and pcDNA-triCEA625-667-IL-18 recombinant plasmid using gene recombinanttechnique.C57BL/6 mice were immunized intramuscularly with the co-expressing DNA vaccine.The mice survival time and tumorsize were measured and the specific CTL cytotoxicity activity which splenic lymphocytes were collected from immunized mice asthe effector cells and tumor cells TC1-CEA+as the target cells was detected by125I-UdR release method.
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