As a strategy of improving the effective utilization of pesticides, Systemic distribution in a target plant is a desirable property that can be achieved after foliar application or root uptake.In our previous work, the long-distance root-to-shoot transport ofN-[3-cyano-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-4-[(trifluoromethy l)sulfinyl]-1H-pyrazol-5-yl]-1-(β-D-glucopyranosyl)-1 H-1,2,3-triazole-4-methanamie (GTF) in the transgenic soybean composite plants transformed by RcHEX3 indicated that RcHEX3 increased the root uptake and the xylem loading of GTF.In this study, a fluorescent GTF derivative, FPGN, was used to visualize the root uptake and the transport pathway of GTF by confocal laser scanning microscopy.Different parts were selected to microscopy(Fig.1).The result shows that FPGN was mainly present in the xylem and the pericycle in the sterile hairy roots of transgenic soybean(Fig.2).