A new bio-inspired method was applied in this study to simultaneously improve the monovalent anion selectivity and antifouling properties of anion exchange membranes (AEMs).Three-layer architecture was developed by deposition of polydopamine (PDA) and electro-deposition of N-O-sulfonic acid benzyl chitosan (NSBC).The innermost and outermost layers were PDA with different deposition time.The middle layer was prepared by NSBC.Fourier transform infrared spectroscopy and scanning electron microscopy confirmed that PDA and NSBC were successfully modified on the surfaces of AEMs.The contact angle of the membranes indicated an improved hydrophilicity of the modified membranes.A series of electrodialysis experiments in which Cl-/SO42-separation was studied, demonstrating the monovalent anion selectivity of the samples.The Cl-,/SO42-permselectivity of the modified membranes can reach up to 2.20, higher than that of the commercial membrane (only 0.78) during 90 minutes in electrodialysis (ED).The increase value of the resistance of the membranes was also measured to evaluate the antifouling properties.Sodium dodecyl benzene sulfonate (SDBS) was used as the fouling materials in ED process and the membrane area resistance increase value of modified membrane was only 0.08 Ωcm2 30 minutes later.Whats more, the membrane resistance increase value of the modified membrane was half of the commercial membrane after 150 minutes.