【摘 要】
：
Selenoprotein W (Selw) plays crucial roles in skeletal muscle.In the present study, we examined the mRNA expression levels of selenoproteins following the silencing or overexpression of Selw in chicke
【机 构】
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Department of Veterinary Medicine , Northeast Agricultural University , Harbin 150030, P.R.China
【出 处】
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中国畜牧兽医学会家畜内科学会2014年学术研讨会
论文部分内容阅读
Selenoprotein W (Selw) plays crucial roles in skeletal muscle.In the present study, we examined the mRNA expression levels of selenoproteins following the silencing or overexpression of Selw in chicken myoblast.The results showed that silencing of Selw increased Gpx3, Gpx4, Txnrd1, Selt, Selh and Sepp1 (P < 0.05), but reduced Sels and Sep15 (P < 0.05).And Reactive oxygen species (ROS) scavenger, N-acetyl-L-cysteine (NAC), influence the effect of Selw on the expression of some selenoproteins (Gpx3, Gpx 4, Txnrd1, Selt, Selh,P > 0.05).However,the overexpression of Selw increased the expression of Sepn1, Selt, Selh, Selm, Selpb and Sepxl (P < 0.05), but decreased Gpx1 (P < 0.05).In addition, H2O2 increased the expressions of selenoproteins (P < 0.05), except the decreased Gpxl and Selw (P < 0.05).And this response was influenced by the overexpression of Selw.The results showed that as one highly expressed selenoprotein in myoblast, variation of Selw influence the expression of other selenoproteins.It indicated that under oxidative condition, myoblast consumed Gpx1 and Selw and increased other selenoproteins to improve the oxidative imbalance.And Selw may regulate some selenoproteins (Gpx3, Gpx 4,Txnrd1 ,Selt ,Selh) by the pathway of ROS.It indicated that Selw reserves irreplaceable roles in myoblast.
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