As an important energy-efficient and environmentally friend separation process, membrane separation has been widely applied in various fields, such as gas separation, water treatment, solvent purification, special separation processes and etc.Metal-organic frameworks (MOFs) are kinds of crystalline materials, which possess a series of extraordinary features, such as uniform pore sizes, large surface areas, large diversity in structures and special adsorption abilities, etc.MOF membranes can offer unique opportunities to overcome the limitations of Robesons "upper-bound" in membrane-based separation, and therefore have received considerable attention which hold great potential to break through most of existing bottlenecks.Various substrates have been employed to support MOF membranes for separation applications.Among them, inorganic substrates, such as metal net, aluminium oxide and titanium dioxide, are mostly studied.Since polymeric membranes and biomaterials have the advantages of low cost,high processing ability and large membrane areas compared with inorganic substrates, the polymeric membranes as substrates will be of good benefit to industrial applications.So far, there are only a few reports concerning the successful MOFs growing on polymer or biomaterial surface.In our research, we have focused on developing serials of MOF/polymer/biomaterial composite membranes with comparative separation performance.To overcome the issues of coverage, adhesion, compression, chemical resistance and swelling in fabrication of MOF composite membrane, the substrates were modified or biomaterials were applied directly to fabricate continuous MOF composite membranes.Furthermore, the mixed matrix membrane and continuous MOF layer were also integrated as well as the continuous MOF layers were in situ transformed to further improve the separation performance of the prepared membranes.