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To acquire the effectiveness of orally treatments, aqueous extracts of Anodonta woodiana (HB) were incorporated into liposome, and evaluated its antitumor activities in vivo (in vivo).The HB-loaded liposomes (HBL) were prepared at the mean size of 14.85μm by reverse-phase evaporation method.Referring to the maximum tolerated doses test, mice orally administrated HBL at 3g/kg body weight dosage, showed no obvious acute toxic sign.Furthermore, the tumoricidal activities of HBL against C26 murine colon carcinoma, Lewis lung tumor, human QGY hepatic carcinoma and MNK-45 (MKN-45) gastric tumor were examined, respectively.In contrast to free HB, HBL possessed remarkable antitumor activity.Simultaneously, the effect of HBL was observed in a dose-dependent rmanner.For C26 murine colon carcinoma and Lewis lungtumor, the inhibitory rates (inhibitory ratios) of HBL were 54.36% and 51.97% at a dose of 400mg/kg, respectively.The suppression of tumor growth and the reduction in body weight were more pronounced in human QGY hepatic carcinoma and MNK-45 (MKN-45) gastric tumors inoculated mice by treatment of HBL during 30 days.All these promising results implied the liposome-incorporated aqueous extracts of Anodonta woodiana had a more potential application as natural antitumor and immunomodulator formulation.