The characteristics of soil microbes are sensitive indicators of soil fertility.Fertilization can influence soil physiochemical and biological properties.The present study investigated the effect of apple special fertilizer(Patent Number: CN 103880508A),inorganic fertilizer, combination of manure and inorganic fertilizer at the equal N, P and K amount on functional diversity of soil microbial community in 10-year Fuji apple orchard by using the Biolog technique.The diversity indexes were calculated using micro-plate optical density values at 96 h.Substrate richness (S) is the number of different substrates used by the community.