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The chemical composition, molecular structure and dose-effect relationship of phytotoxin of Ginseng Black Spot pathogen was studyed in this paper.The impure phytotoxin was isolated by silica gel adsorption chromatograph and preparative chromatography respectively.The comparative lesser effective section of phytotoxin of Ginseng Black Spot Pathogen strain was finded by biological assay.Composition and molecular structure of the phytotoxin was identified with the method of GC-MS, preliminary definite as butyl benzene o-dicarboxylate.The result of this research reveal the pathogenic mechanism of toxin, established scientific base for purifying toxin of Alternaria panax.