Objective Altered hippocampus is a valuable indicator of possible conversion from amnestic type mild cognitive impairment (aMCI) to Alzheimers disease (AD).However, little is known about the disrupted patterns of hippocampus subregional networks in aMCI subjects.Methods aMCI group-1 (n =26) and controls group-1 (n =18) were underwent baseline and mean 20 months follow-up period for resting-state fMRI scan.Integrity of distributed networks incorporating six hippocampal subregions (i.e.cornu ammonis, dentate gyms and subicular complex, bilaterally) were then explored over time and comparisons made between groups.The ability of these extent longitudinal changes to separate unrelated groups of 30 subjects (aMCI-converters, n =6; aMCI group-2, n =12; controls group-2, n =12) were further assessed.Results Six longitudinal hippocampus subregional networks showed similar changes in aMCI subjects over time, which were mainly associated with medial frontal gyrus, lateral temporal cortex, insula, posterior cingulate cortex (PCC) and cerebellum.Moreover, the disconnection of hippocampal subregions and PCC maybe a key factor of impaired episodic memory in aMCI, and the functional index of these longitudinal changes allowed well classifying independent samples of aMCI converters from non-converters (sensitivity is 83.3%, specificity is 83.3%) and controls (sensitivity is 83.3%, specificity is 91.7%).Conclusion It demonstrated that resting-state functional deficits were in six hippocampus subregional networks associated with no priority in aMCI subjects.However, the combination of these sub-networks could generate superior classifications of the initiation and progression from aMCI to AD.