Whole-genome Resequencing Identify the Seed Coat Color Gene in Dahuang (a landrace of Brassica rapa)

来源 :2016年作物学全国博士生学术论坛 | 被引量 : 0次 | 上传用户:yangchao2005
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  Objectives: A yellow-seeded coat is a desirable agronomic trait in the seeds of oilseed-type Brassica crops.In this study,we identified a candidate gene for seed coat color in Dahuang,a landrace of Brassica rapa.Methods: Whole-genome resequencing with bulked segregation analysis was conducted to identify candidate intervals for the candidate gene Brsc1.A genome-wide comparison of SNP profiles was constructed between yellow-seeded and brown-seeded bulk samples.SNP index analyses identified a major candidate interval on chromosome A9.Results: According to gene annotation,Bra028019 was identified as an important candidate gene for Brsc1 in the overlapping region.Quantitative RT-PCR revealed that Bra028019 mainly functioned in the seed.Point mutations and small deletions in Bra028019 were found between yellow-and brown-seeded Dahuang plants.Conclusion: Whole-genome resequencing identify candidate intervals for Brsc1.According to gene annotation and quantitative RT-PCR revealed that Bra028019 mainly functioned in the seed.Collectively,the expression and sequence analysis results provide preliminary evidence that Bra028019 is a candidate gene for the seed coat color trait in Dahuang.
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