荧光传感器阵列用于同时分析多种生物标志物

来源 :2016中加分析化学研讨会(CCACC2016) | 被引量 : 0次 | 上传用户:wangliang284
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  传感器阵列是通过模拟动物嗅觉/味觉系统建立起来的一种仿生分子识别手段;荧光因其灵敏度高、输出信号丰富等优点成为最广泛应用的信号输出方式。在早期的荧光传感器阵列中,传感单元都是单通道信号输出,因此需要以阵列分离的方式分别采集每一个传感单元的信号,需要多次重复测量并消耗大量的待测物。我们采用具有不同荧光发射波长的传感材料构建传感单元,可以避免传感单元的空间阵列分离。我们首先利用可被DNA自身猝灭的三色荧光染料设计构建了荧光传感器阵列,可用于七种核酸外切酶高灵敏识别与区分。由于多色荧光染料的最适激发波长各不相同,对多通道荧光信号的采集依然需要多个单色激发光源或多个滤光片等复杂昂贵的光学装置。我们进一步构建了只需单个激发波长的多色量子点荧光传感器阵列,可以快速(< 1 min)准确识别数十种血清蛋白和七种细胞。
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