【摘 要】
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In the processes of metabolism,the high energy conversion and great turnover number are exemplified by sequential enzymes catalytic reactions.These phenomena indicate multi-enzymes cascade reactions h
【机 构】
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State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engin
【出 处】
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第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议
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In the processes of metabolism,the high energy conversion and great turnover number are exemplified by sequential enzymes catalytic reactions.These phenomena indicate multi-enzymes cascade reactions have more advantages than single enzyme in the bio-fuel cell,detections fields and modeling reaction pathways within cells1-3,However,the challenge is critical to reveal how biology integrates temporal and spatial information to determine the biological function.What is more,understanding the effect of spatial organization on enzymatic activity in multienzyme systems is not only fundamentally interesting,but also important for translating biochemical pathways to noncellular environments.Here,based on the our pervious research by using Scanning Electrochemical Microscopy (SECM)4,a study of distance-dependence for the activity of β-Galactosidase (β-Gal)/ Glucose Oxidase (GOD) cascade by immobilizing β-Gal/GOD on Polycarbonate (PC)/PDMS hybrid microchip is reported in this paper.A schematic representation of enzymes cascade reaction in microchannel is outlined in Figure 1.The influence of spatial distance and velocity on the kinetic of enzymes cascade reaction was investigated by using electrochemical detection at the end of microchip.A 2D finite element model was applied to understand the effect of mass transport on the spacing-dependent enzymes cascade reaction kinetics.
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