Development of a highly sensitive ELISA for detecting bovine serum albumin (BSA) based on specific p

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【摘要】

：

【作者】

：

Zhang K Song CJ Li YM Li Q Yang K Jin BQ

【机构】

：

Department of Immunology, The Fourth Military Medical University, Xi'an, 710032, China

【出处】

：

全军免疫学第七届学术交流会暨免疫学新进展主题论坛

【发表日期】

：

2009年12期

【关键词】

：

BSA Monoclonal antibody Sandwich ELISA

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　　A highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) for quantifying BSA was successfully developed,based on two mAbs recognizing different epitopes on BSA molecule.Eleven murine hybridomas secreting BSA specific mAb were established by routine B lymphocyte hybridoma technique in which the complete culture medium contained 10% (v/v) horse serum.A pair of mAbs(FMU-BSA NO.6 and FMU-BSA NO.11) were selected for development of sandwich ELISA.

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