【摘 要】
：
Negative bHLH transcription factor Hesl can inhibit neural stem cells (NSCs) from precocious neurogenesis through repressing proneural gene expression; therefore,sustenance of Hesl expression is cruci
【机 构】
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Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Aca
【出 处】
：
中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
论文部分内容阅读
Negative bHLH transcription factor Hesl can inhibit neural stem cells (NSCs) from precocious neurogenesis through repressing proneural gene expression; therefore,sustenance of Hesl expression is crucial for NSC pool maintenance.Here,we find that Ids,the dominant-negative regulators of proneural proteins,are expressed prior to proneural genes and share an overlapping expression pattern with Hes 1 in the early neural tube of chick embryos.Overexpression of Id2 in the chick hindbrain upregulates Hesl expression and inhibits proneural gene expression and neuronal differentiation.By contrast,Hesl expression decreases,proneural gene expression expands and neurogenesis occurs precociously in Idl; Id3 double knockout mice and in Idl-3 RNAi-electroporated chick embryos.Mechanistic studies show that Id proteins interact directly with Hes 1 and release the negative feedback autoregulation of Hes I without interfering with its ability to affect other target genes.These results indicate that Id proteins participate in NSC maintenance through sustaining Hes 1 expression in early embryos.
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