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Real-time quantitative PCR (qRT-PCR) has emerged as a powerful and popular tool for quantitating differences in transcriptional gene expression levels between samples.Validation of the stability of reference genes is a fundamental step prior to initiating qRT-PCR assays.Sparassis latifolia (S.latifolia) is an edible and medicinal fungus contained a remarkably high concentration of β-glucan,which has many biological and pharmacologic activities.S.latifolia can be a model strains for study fungal photobiology,as its fruit body formation need more light than other fungi.However,its suitable reference genes have not yet been determined.In the present study,10 candidate reference genes in S.latifolia were evaluated and validated under different developmental stages and light conditions.To evaluate the suitability of candidate reference genes,two software-based approaches (geNorm and NormFinder) were used to analyze the tested genes.According to our results,GAPDH and Actin were expressed at the most stable levels under different developmental stages and light conditions.