【摘 要】
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Objective: To develop a new mirco-straw (named as LSL straw) for cryopreservation of few sperm retrieved from severeoligozoospermia and testicular biopsy samples.Methods: We developed a new micro-stra
【机 构】
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Department of Urology, Renji Hospital, Affiliated to Shanghai Jiao Tong University School of Medicin
【出 处】
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GTAUF2014 & APSMHA2014第六届长城泌尿男科转化医学论坛暨第七届亚太男性健康与抗衰老研究学会年会
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Objective: To develop a new mirco-straw (named as LSL straw) for cryopreservation of few sperm retrieved from severeoligozoospermia and testicular biopsy samples.Methods: We developed a new micro-straw with max volume of 50 μL.The same sperm samples were cryopreserved using new mirco-straw and conventional 0.2 and 0.5 mL straws and post thaw motility, acrosomal integrity and sperm DNA fragmentation were compared.Total of 32 semen samples were used in this study and each sample was diluted to sperm concentration 10×106/mL.The diluted semen was mixed with cryoprotectant in ratio of 1:1 before transferring to micro-straw and 0.5 and 0.25 mL straws.
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