【摘 要】
：
High prevalence of multidrug-resistant bacteria decreases effectiveness of conventional antibiotics and become severe threat to public health.New improvements in current methods or innovative therapeu
【机 构】
：
Department of Pharmacology,School of Pharmacy,Fourth Military Medical University,Xi'an,shaanxi,7100
【出 处】
：
2012年中国药学大会暨第十二届中国药师周
论文部分内容阅读
High prevalence of multidrug-resistant bacteria decreases effectiveness of conventional antibiotics and become severe threat to public health.New improvements in current methods or innovative therapeutic strategies are urgently needed to solve this problem.Antimicrobial peptides,known as host defense peptides,have been widely accepted as the first line to defend microbial infection effectively.However,low selectivity and high-cost are major impediment in its clinical applications.In this study,a novel derivative peptide (A-TH-PG) was produced by a γ-core—substituted design strategy.A-TH-PG not only retains the low toxicity of the advantages of the thanatin,but also reduces the synthesis costs.Properties of little hemolysis and relatively high stability in plasma were confirmed.A-TH-PG is highly effective against MRSE in vitro,and excellent in vivo therapeutic effects were also observed in a septicemic animal model.Combined mechanistic investigations through scanning and transmission electron microscopy and fluorescence assays,revealed that the killing of Methicillin-resistant Staphylococcus epidermidis (MRSE) was caused by disrupting cell division severely and damaging cell membranes.Furthermore,it is speculated that the disturbance of cell division may be a key biological action of A-TH-PG.Our results offer a feasible strategy for treating multidrug-resistant infections.
其他文献
目的 研究血塞通配合血氧疗法治疗椎基底动脉供血不足时脑血流动力学和血液黏度的变化。方法对20例椎基底动脉供血不足思者血塞通配合血氧疗法治疗前及治疗2周后分别行经颅多普勒和血液流变学检测，观察症状和体征变化。结果血塞通配合血氧疗法治疗后椎基底动脉系统血流速度增快。血液黏度降低。症状和体征缓迅速。结论血塞通配合血氧疗法能改善椎基底动脉供血不足患者的脑部血液循环。治疗椎基底动脉供血不足安全、见效快。
目的：建立同时测定人血浆中贝那普利及贝那普利拉浓度的UPLC-MS/MS法,研究中国健康志愿者单次口服氨氯地平贝那普利胶囊后贝那普利及其活性代谢物贝那普利拉的药动学特征.方法：24 名健康受试者随机分成3 组,每组8 人,男女各半,分别单次口服贝那普利含量为10、20、30mg 氨氯地平贝那普利胶囊.血浆样品用乙腈进行蛋白沉淀,贝那普利及贝那普利拉的血浆浓度采用超高效液相色谱-串联质谱法检测.结果
目的 合成新型类黄酮化合物并探究其生物活性。方法 以杜鹃素为先导，对其B环和C环进行结构改造和修饰。结果 合成了二氢黄酮、查尔酮、橙酮以及二氢异黄酮等四类新型类黄酮化合物84个，考察了化合物的抗肿瘤活性、酪氨酸蛋白激酶抑制活性、血管内皮细胞保护活性和血管平滑肌细胞增殖抑制活性。结论 部分目标化合物具有良好的生物活性。
我们研制了新型鼠疫基因工程疫苗,疫苗是由鼠疫菌培养液中提取天然F1 抗原与重组鼠疫菌rV 抗原配伍,以氢氧化铝为吸附剂而组成的基因工程疫苗,对疫苗的各种理化指标,按“《中国药典》2005年版二部原料药与药物制剂稳定性试验指导原则”进行检定,各项指标均符合规程要求。鼠疫疫苗属对温度特别敏感的药物制剂,本文重点讨论对临床试验申请用样品鼠疫疫苗20081002,20081003,20081004 三批制
Safe and effective gene delivery is essential to the success of gene therapy.We synthesized and characterized a novel nonviral gene delivery system in which folate (FA) molecules were used as stoppers
目的：比较美国药典(USP35)和中国卫生部颁标准中氨磷汀主体和相关物质的HPLC测定法.方法：USP35版采L7色谱柱,用甲醇：0.94g/L己烷磺酸钠水溶液(磷酸调至pH3.0)=7：18为为流动相,流速为1.0mL/min,检测波长220nm；中国卫生部颁布的标准则采用L1色谱柱,用甲醇：3.5mmol/L辛烷磺酸钠水溶液(磷酸调节至pH3.0)=1：1为流动相,流速为0.7mL/min,检
目的 在无催化剂条件下，开展氢氧化镁促进的芳香胺化反应研究。方法 在氢氧化镁体系下，通过芳香亲核取代机理使芳香(杂)环氯化物与多种胺发生C-N键的偶联反应。结果 成功合成出了十二种预期产物，反应的产率在37～99％之间。结论 在无催化剂存在下，氢氧化镁可以促进C-N键的偶联反应，是一种新的合成方法。
目的：建立用高效液相色谱法测定七叶灵颗粒中特女贞苷和柚皮苷含量的方法.方法：使用Kromasil C18色谱柱(250×4.6mm,5μm),流动相为乙腈-4％冰醋酸(17：83,V/V),检测波长为224nm,流速：1mL·min-1,柱温30℃,特女贞苷线性范围为0.04～1μg(r=0.9999),柚皮苷线性范围0.08～2μg(r=0.9999),特女贞苷精密度RSD=2.28％(n=5)