论文部分内容阅读
Vibrio parahaemolyticus is one of the typical zoonotic pathogens worldwide that causes acute gastroenteritis in humans and vibriosis in many marine fishes,shrimps,shellfishes,crabs and other aquatic animals.Zebrafish larva represents a useful in vivo model for identification of innate host determinants of response to bacterial infection owing to the clear separation of innate immunity from adaptive responses.However,the mechanism of the innate immune response infected by V.parahaemolyticus in the zebrafish larva is lacking.To well understanding the zebrafish innate immune response,we used RNA-Seq by Illumina Hiseq 4000 to analyze the transcriptomic profile by V.parahaemolyticus strain vp13 immersed infection.In this study,gene expression comparison revealed that 31,949 genes were detected,and 602 differentially expressed genes(DEGs)were identified by the comparison among all of the libraries,in which 427(70.93%)genes were up-regulated and 175(29.07%)genes were down-regulated.The quantitative gene expression analysis of significant DEGs,such as Il-11a,ccl 20a,tnf a,mmp 13,hsp 70 and cxcl 8a were performed.Our results provide a useful reference for future analysis of signal transduction pathways underlying the systemic innate immune response to external bacteria and secreted virulence factors and toxins.Moreover,the results obtained in the present study clearly demonstrated that immersion of 3 dpf larva in Vp13 for 2 hours doesn`t lead to widely responses of TLR signaling pathways.But complement and coagulation cascades,chemokine signaling pathway,systemic lupus erythematosus and leukocyte transendothelial migration can be activated to resist the V.parahaemolyticus invading.In conclusion,the data obtained in this study provide a valuable resource for future genetic and genomic research on zebrafish and closely related species,and may also contribute to the investigation of candidate genes in zebrafish immunity.