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Moso bamboo [Phyllostachys heterocycla var.pubescens (Mazel ex J.Houz.) Ohwi] is one of the most important forest crops in both Asia and China.Although there are many sympodial bamboo tissue culture protocols established, there is no available protocols for monopodial bamboos, such as Moso bamboo.In this report, embryogenic callus induction, somatic embryo development, and somatic embryo germination was established for Moso bamboo from zygotic seed embryos.Callus was initiated from zygotic embryos after 10-20 d culture on MS media supplemented with 4 mg/L 2,4-D and 0.1 mg/L zeatin (ZT).Nearly 47% of the explants produced calli, with nearly 15% of the calli embryogenic in nature.These calli can be subcultured for proliferation in the same medium and maintained their regeneration capacity after one year of subculture.The viable somatic embryoids could be regenerated in medium containing ZT.Nearly 5% of the calli could subsequently be regenerated into plantlets.The root growth was increased when the plantlets were transferred to medium containing 2mg/L NAA.After 15 days of subculture, the plantlets could be transferred to the greenhouse.