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Momordica charantia, called bitter melon or bitter gourd, has been reported to have detoxification, hypoglycemic, antiviral, antidiabetic, and antitumor activities.The chemical constitution of Momordica charantia is very complex.Therefore, quality control technique of such natural products encounters more and more challenges.Fingerprint can reflect the overall information of complex samples and has been widely used as an effective quality assurance method.High performance liquid chromatography (HPLC) fingerprint is preferred method and has inherent advantages over spectroscopic or other chromatographic fingerprint.