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The aim of this research was to study the short-term effects of different nitrogen concentrations on the growth rate,photosynthesis and fatty acid composition of the diatom Nitzschia closterium f.minutissima,one of the most dominant species in South China Sea.Cells were collected during the logarithmic phase and exposed to five concentrations of nitrogen (NaNO3) : 75mg/L,15mg/L,5mg/L,3.75mg/L and 3mg/L).