The wide application of carbon nanomaterials in various fields urges in-depth understanding of the toxic effects of these materials on biological systems and the mechanisms.Cell autophagy was recently recognized as an important lysosome-based pathway of cell death and autophagosome accumulation has been found to be associated with the exposure of various nanoparficles, but the underlying mechanisms are still uncertain due to the fact that autophagosome accumulation can result from autophagy induction and/or autophagy blockade.