c-Met overexpression promotes reendothelialization and inhibits neointimal formation after balloon i

来源 :第十二届中国介入心脏病学大会(CIT2014) | 被引量 : 0次 | 上传用户:fw1989
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Background To explore the effect of c-met overexpression in EPCs on reendothelialization after balloon injury Methods EPCs derived from mouse bone marrow were isolated and cultured.3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays were used to evaluate EPC proliferation.Adenoviral vector expressing c-Met was generated using the AdEasy system.To evaluate the role of HGF/Met in vascular repair in vivo,we used balloon-injured rat carotid artery model.Evans Blue dye was administered to evaluate reendothelialization after 10 days injury,and the neointimal formation was assessed at 21 days following vascular injury.
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