Background & Aims Glypican 3 (GPC3) expresses abundantly in fetal liver and is silent in normal adult liver but becomes reactivated in hepatocellular carcinoma (HCC).And recently GPC3 has been the focus of particular interests because of its potential usefulness as a specific marker for HCC.To study the mechanisms regulating the reactivation of GPC3 in HCC appears to be clinically meaningful.Zinc-fingers and homeoboxes 2 (ZHX2) are ubiquitously expressed and localized to the nuclei of cells,and appear to function as transcriptional repressors.Previous data have shown that ZHX2 protein is involved in postnatal repression of GPC3 inmice.Here,we hypothesized ZHX2 might regulate GPC3 expression in HCC.