This paper introduces a nano-CEEC chip incorporating nano-electrodes with sequential applied electric fields to pre-concentrate scarce neurotransmitters released from PC-12 cells for continuous detection.This device provides a novel platform for single cell-cell interaction studies and a bioelectronic interface for fundamental understanding of single-cell interactions.Various methods for high sensitive detection of neurotransmmiter have been reported.One method [1] employing Surface Enhanced Resonance Raman Scattering (SERRS) to detect dopamines approached pM level without any pretreatment in short data acquisition time.On the other hand,carbon-fiber-microelectrode arrays were utilized to electrochemically image neurochemical secreted from individual PC-12[2] in 2D manner.However,without pretreatment and pre-concentration,high sensitive detection of biomolecules can still encounter many non-specific binding and interference issues.Therefore,the integration of online sampling to gather almost all of the molecules released from single cells and perform sample pre-concentration immediately is necessary before high sensitive detection.In this paper,we introduces a Nano-CEC chip integrates three crucial subunits to detect the scarce neurochemicals,including a serial of electrodes operated in different time to perform sample concentration; a dual-asymmetry electrokinetic flow device for sample stacking as well as pre-concentration in nanochannel,and an electrochemical detector (Figure 1).The most important part is a serial of electrodes for the sample-preconcentraion,and a schematic view of the mechanism of restacking is shown in Figure 2.Each of four electrodes is compose of silicon nanowires,and then passivated by SiO2 layer.When electric fields applied onto the nanowires,the polarization appears in the insulate layer and pre-concentration of biomolecules occurs in front of the filter.After the injection of sample containing dopamine,electric fields are applied to the four electrodes for after 10-minute-preconcentation and then the signal changes can be recorded at every 10 s.A detection limit of 30~75 zeptomoles was achieved,which is close to the levels released by a single neuron in vitro.