Although electric vehicle(EV)has been regarded as a promising method to reduce tailpipe emissions and energy consumption,the mixed traffic flow of EVs and internal combustion engine vehicles(ICEVs)make the energy/emission reduction objective more difficult,because EVs and ICEVs have general various acceleration/deceleration behaviour.This paper propose a low emission oriented speed guidance(SG)model to address the energy/emission reduction issue for mixed traffic flow at an isolated signalized intersection,which focus on the effect of queue and resulted stop-and-go behaviour.The totally energy/emission under different EV market penetration rates with various traffic volumes are analysed and compared.Numerical examples demonstrate that the proposed SG model has better performance than the conventional models without considering the impact of the queue.With a certain traffic volume,the energy/emission reduction effects under speed guidance will increase with the share of EV.