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Objects:The purpose of this study is to identify serum biomarkers which can assist the neuroimaging evaluations to diagnose acute ischemia stroke.Methods:Six cases of AIS with hypertension were selected and matched with six healthy controls.lsobaric tags for relative and absolute quantitation(iTRAQ)coupled with MS used to compare and analyse differential protein of these 12 samples respectively.Enzyme linked immunosorbent assay(ELISA)validation was undertaken in 25 independent samples.Results:In total,242 proteins were identified from 49297 spectras using iTRAQ coupled with MS.Compared with the healthy control group,there were 14 differentially expressed serum proteins,13 0f them were up-regulated,and one of them was down-regulated.Furthermore,CST3 and PCYOXl were used to carry out the validation of western blot,and the results were consistent with the result of iTRAQ coupled with MS.Conclusion:KIC9,APOH,PCYOXl,IGJ,PIGR may be novel and potential biomarkers for early diagnosis of AIS.