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  5. A New Set of EST-SNP for the Implementation of a Garden Asparagus Genetic Map

A New Set of EST-SNP for the Implementation of a Garden Asparagus Genetic Map

来源 :XIIIth International Asparagus Symposium(第十三届世界芦笋大会) | 被引量 : 0次 | 上传用户：alpaalpa
【摘 要】
：
Single nucleotide polymorphisms (SNPs) are uniformly distributed molecular markers in plant genomes.Even though high throughput platforms have been developed in several important crops to identify SNP
【作 者】
：
Francesco Mercati Paolo Riccardi Caterina Longo Rick Berentsen Agostino Falavigna Francesco Sunseri 
【机 构】
：
Dipartimento di Agraria, Università Mediterranea di Reggio Calabria, Salita Melissari,89124 Reggio C
【出 处】
：
XIIIth International Asparagus Symposium(第十三届世界芦笋大会)
【发表日期】
：
2013年4期
【关键词】
：
molecular markers Next Generation Sequencing (NGS) eDNA library genetic map 
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　　Single nucleotide polymorphisms (SNPs) are uniformly distributed molecular markers in plant genomes.Even though high throughput platforms have been developed in several important crops to identify SNP markers, garden asparagus (2n=2x=20) breeding has been limited by a low number of such markers.In this study, Next Generation Sequencing (NGS) technology has been used to sequence and compare transcriptomes from two garden asparagus (Asparagus officinalis L.) genotypes: "1770" a double haploid (DH) male clone and "G190" a heterozygous female clone.Over 1,800 putative SNPs were detected [1], 288 were validated using the Illumina BeadXpress assay.
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