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The purpose of this study is to develop a novel microsphere with efficiently hemostatic property from chitosan and gelatin using appropriate amount of genipin as a crosslinker.The size of chitosan/gelatin crosslinked microspheres(Cs/Gel MPs)ranged from 20 μm to 80 μm.The performance of these Cs/Gel MPs were evaluated with experiments in vitro and in vivo.Results in vitro showed that the best hemostatic efficacy was achieved by Cs/Gel-0.75 MPs.