Development of In situ hybridization and real-time PCR assays for the detection of Hepatospora erioc

来源 :第十届世界华人虾蟹养殖研讨会暨2016年中国河蟹产业发展高峰论坛 | 被引量 : 0次 | 上传用户:cjwxwq
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  A microsporidian parasite,Hepatospora eriocheir,is an emerging pathogen for the Chinese mitten crab Eriocheir sinensis.Currently,there is scant information about the way it transmits infection in the crustacean of commercial importance,including its pathogenesis,propagation and infection route in vivo.In the present study, chromogenic in situ hybridization (ISH) and quantitative real-time PCR (qPCR) assays were developed to address this pressing need, and we provided an advance in the detection methods available.
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