【摘 要】
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This study describes the potential use of attenuated Salmonella enterica serovar Typhimurium Strains (S.typhimurium) to express and deliver a L7/L12 and BLS fusion antigen of Brucella as a vaccination
【机 构】
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Immunology department of Institute of Microbiology and Epidemiology, Academy of Military Medical Sci
【出 处】
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全军免疫学第七届学术交流会暨免疫学新进展主题论坛
论文部分内容阅读
This study describes the potential use of attenuated Salmonella enterica serovar Typhimurium Strains (S.typhimurium) to express and deliver a L7/L12 and BLS fusion antigen of Brucella as a vaccination strategy to prevent Brucella infection in mice.S.typhimurium X4072 that contained a pTrc99A-BLS-L7/L12 plasmid,designated X4072bl,can deliver a L7/L12 and BLS fusion antigen expressed by the bacterium itself,while S.typhimurium X4550 that contained an asd-pVAX1-BLS-L7/L12 (asd-pBL) plasmid,designated X4550bl,can deliver the antigen to be expressed in target eukaryotic cells.When orally administered to BALB/c mice,both attenuated carrier strains were able to elicit mucosal and systemic immunity,which induced protection against B.abortus 544 infection in mice.
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