Background:According to the world health organization(WHO), China is one of 22 countries with serious tuberculosis (TB)infection and one of the 27 countries with serious multidrug-resistant to TB .Despite the decline of tuberculosis in the overall population, healthcare workers (HCWs) are still at high risk of infection. In comparison with high-income countries, TB prevalence among HCWs has been higher in low- and middle-income countries. Low-dose computer tomography (LDCT) is becoming more popular due to its superior sensitivity and lower radiation dose. However, there is no report about active pulmonary TB among HCWs scanned by LDCT. The purpose of this study is to study the TB infection status in HCWs of hospitals specializing in TB treatment and to explore the significance of LDCT in these workers. Methods: This study retrospectively analyzed the health physical examination data of 1012 healthcare workers in Beijing chest hospital, from September 2012 to December 2015.Low-dose lung CT examination were performed in all cases. The comparisons between active and inactive PTB for the CT findings were made by using the Pearson chi-square test or Fishers exact test. Results: During 4-year medical examination, active tuberculosis were found in 19 cases (1.9%) and inactive tuberculosis were found in 109 cases. The prevalence rate of active tuberculosis in HCWs for each year were 1.24%, 0.67%, 0.81%, and 0.53% , respectively from 2102 to 2015. The incidence rate of active tuberculosis in tuberculosis hospital staff were 0.86%, 0.41%, 0.54%, and 0.26%. Majority of HCWs infected with active TB(78.9%,15/19) worked in the high-risk areas in the hospital. Comparison of the CT signs between active and inactive groups by chi-square test show that tree-in-bud, cavity, fibrous shadow, and calcification had significant difference (P=0.000, P=0.021, P=0.001, P=0.024). Tree-in-bud and cavity suggest active pulmonary tuberculosis, whereas fibrous shadow and calcification are the main features in inactive pulmonary tuberculosis Conclusion: Healthcare workers in tuberculosis hospital are high-risk groups of active tuberculosis infection, especially in HCWs worked in the high-risk areas in the hospital. LDCT examination at each year in the high-risk groups is feasible and necessary.