【摘 要】
：
Objective Long-term excessive sodium fluoride (NaF) intake can cause many bone diseases and non-skeletal fluorosis.The kidneys are the primary organs involved in the excretion and retention of NaF.The
【机 构】
：
Laboratory Animal Center, Shanxi Key Laboratory of Experimental Animal Science and Animal Model of H
论文部分内容阅读
Objective Long-term excessive sodium fluoride (NaF) intake can cause many bone diseases and non-skeletal fluorosis.The kidneys are the primary organs involved in the excretion and retention of NaF.The objective of the present study was to determine the effects of NaF treatment on renal cell apoptosis,DNA damage,and the protein expression levels of cytosolic Cyt C (Cytochrome C),cleaved caspase-9,-8,-3 in vivo.Methods Male Sprague-Dawley rats were divided randomly into four groups (control,low fluoride,medium fluoride,and high fluoride) and administered 0,50,100,and 200 mg/L of NaF,respectively,via drinking water for 120 days.Histopathological changes in the kidneys were visualized using hematoxylin and eosin staining.Renal cell apoptosis was examined using flow cytometry,and renal cell DNA damage was detected using the comet assay.Cytosolic Cyt C and cleaved caspase-9,-8,-3 protein expression levels were visualized using immunohistochemistry and Western bloting.Results The results indicated that NaF treatment increased apoptosis and DNA damage.In addition,NaF treatment increased the protein expression levels of cytosolic Cyt C,cleaved caspase-9,-8,-3.ConclusionsThese results indicated that NaF induces apoptosis in the kidneys of rats through caspase mediated pathway and DNA damage may be in involved in this process.
其他文献
目的 研究纤维素含量对诸氏鲻虾虎鱼幼鱼生长的影响，为配合饲料的开发及相关标准的制定提供依据.方法 配制5种不同纤维素含量的配合饲料，饲喂60日龄幼鱼8周，测定存活率(SR)、增重率(WG)、特定生长率(SGR)、摄食率(FR)、饲料系数(FCR)和肥满度(FUL)，切片观察各组虾虎鱼的肠道，并测定鱼体基本组分(粗蛋白、粗脂肪、水份和灰分).结果 幼鱼SR随纤维素含量的增加逐渐下降；WG和SGR先上
目的 考察番茄红素蜂胶胶囊对去势雄性大鼠前列腺增生的改善作用.方法：雄性去势SD大鼠,皮下注射丙酸睾丸素建立前列腺增生模型,灌胃给予番茄红素蜂胶胶囊,考察其对大鼠前列腺湿重,前列腺体积,SOD、MDA水平及前列腺组织结构的影响.结果：番茄红素蜂胶胶囊高剂量能降低前列腺增生大鼠的前列腺指数(PI)及前列腺增生率(P＜0.05)并提高组织中SOD活力(P＜0.05).结论：受试物番茄红素蜂胶胶囊具有改
Objective The increasing application of silver nanoparticles (AgNPs) has been raising concerns about their potential adverse effects to human and the environment.However,the knowledge on the systemic
Chitosan-based tissue engineered nerve grafts are successfully used for bridging peripheral nerve gaps.The biodegradation products of chitosan are water-dissolvable chitooligosaccharides (COSs),which
目的 通过研究芒果苷联合紫杉醇对前列腺癌细胞增殖及转移的影响,初步探讨芒果苷是否具有逆转前列腺癌细胞耐药的作用.方法 体外实验培养前列腺癌细胞PC3,DU145及其紫杉醇耐药细胞PC3-TXR,DU145-TXR.利用细胞增殖实验(MTS方法)检测芒果苷对PC3,DU145,PC3-TXR, DU 145-TXR细胞增殖的影响,分别设空白组,七个浓度的芒果苷组(2.5,3.75,5,7.5,10,
Schisandrin B (Sch-B),the most abundant active ingredient of the fruit of Schisandra chinensis,has been proposed to have antioxidant,anti-tumor and anti-inflammatory effects.The present study was unde
Objective studies have demonstrated that 2-dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione (DMDD),isolated from the roots of Averrhoa carambola L.,has significant therapeutic potential for the treatmen
目的 研究吊竹梅水提物(AEZPS)对糖尿病小鼠肾脏的保护作用.方法 采用尾静脉注射链脲佐菌素(STZ)建立糖尿病模型,然后随机分组：模型组、吊竹梅提取物低、中、高剂量组(相当于生药10,20,40 g·kg-1),每组10只.同时,另设10只健康小鼠作为正常对照组,模型组与正常组ig双蒸水(20ml·kg-1).ig给药28d,测定空腹血糖(FBG)、血尿素氮(BUN)、血肌酐(Scr)和血脂.
目的 比较脐带间充质干细胞移植对双胎脑瘫患儿运动功能的影响,探讨疗效与遗传因素的相关性。方法：招募8对(16例)双胞胎脑瘫患儿,应用异体脐带间充质干细胞行蛛网膜下腔注射疗法,于治疗前及治疗后1个月、6个月进行粗大运动量表(GMFM,gross motor function measure)和精细运动量表(FMFM,fine motor funetion meaure)测试,将结果进行自身前后比较,
目的 体外培养骨髓脂肪祖细胞(BMAPs),比较其与骨髓间充质干细胞(BMSCs)在形态、表型特征、成脂肪分化能力以及脂肪分化相关基因表达方面的差别.方法 确无菌取1～2月龄雄性SD大鼠的股骨和胫骨骨髓制作细胞悬液,EBM-2(含20％FBS、15μg/ml ECGs)培养基差速贴壁法培养第三次贴壁细胞,取3到5代细胞用于实验.流式细胞分析检测CD34、CD44、CD45以及CD90表达；STEM