【摘 要】
：
生长素在植物生长发育中发挥重要调节作用,其主要通过生长素受体TIR1介导负调控因子AUX/IAAs的降解,进而释放ARFs转录因子调控下游基因表达.泛素/蛋白酶体(Ubiquitin-Pro
【机 构】
：
中国科学院上海生命科学研究院植物生理生态研究所分子遗传国家重点实验室,上海200032
论文部分内容阅读
生长素在植物生长发育中发挥重要调节作用,其主要通过生长素受体TIR1介导负调控因子AUX/IAAs的降解,进而释放ARFs转录因子调控下游基因表达.泛素/蛋白酶体(Ubiquitin-Proteasome)途径可以有效快速地选择性的移除相关蛋白,在激素信号响应、发育及胁迫响应等过程中发挥重要作用.虽然已有研究表明了蛋白酶体在生长素信号中的重要作用,但是其调控机制以及AUX/IAA降解的平衡机制目前仍不清楚.我们鉴定了一个动物中蛋白酶体活性抑制蛋白PI31的同源基因,PTRE1 (PROTEASOME REGULATOR 1),其编码一个脯氨酸寓集蛋白.功能研究表明PTRE1介导了生长素调节的蛋白酶体活性过程并协调TIR1介导的蛋白降解以调控生长素信号.PTRE1定位于细胞膜、细胞核及内质网,生化分析表明PTRE1可以促进26S蛋白酶体活性.突变体ptre1表现出多重发育缺陷并对生长素响应不敏感；遗传研究表明TIR1与PTRE1具有协同效应,ptre1突变体中TIR1介导的AUX/IAA降解减弱,同时生长素可以通过PTRE1抑制蛋白酶体活性.进一步的研究表明生长素调控了PTRE1在细胞膜及核中的分布从而调控其活性.这些结果为了解AUX/IAA的降解调控及生长素作用机制提供了新的线索.
其他文献
棉属叶形呈现多样化,根据棉花叶裂的深浅可以分为阔叶和鸡脚叶,鸡脚叶又可以分为海岛型鸡脚叶、亚鸡脚叶、鸡脚叶和超鸡脚叶。阔叶和鸡脚叶由位于D1染色体L2基因座上的等
Populus and Salix are sister genera in the Salicaceae family.In both lineages extant species are predominantly diploid.Genome sequencing of these two lineag
The worlds commercial production of natural rubber,an essential industrial raw material,is solely from one tropical tree species,Hevea brasiliensis (para ru
The classic phytohormones play important roles in regulation of plant stem cells,and exhibit complex functional interactions.Among them,auxin was previously
Brassinosteroids (BRs) are steroid plant hormones that regulate many developmental and physiological processes in pants including responses to stress.Howeve
The antagonistic crosstalk between gibberellic acid (GA) and abscisic acid (ABA) plays a pivotal role in modulation of seed germination.However,the molecula
Many studies have been dedicated to the indispensable roles of jasmonate in plant growth and defence,but the molecular mechanisms preventing the overproduct
Auxin plays an important role to mediates endogenous developmental signals and environmental cues and thus control root growth plasticity.Aluminum stress,wh
A major progress in our understanding of the jasmonate signaling is that the perception of the active hormone by the E3 ubiquitin ligase SCFCOI1 is tightly