object:To observe the promoting concrescence of diabetic wound effect of MSCs using two kinds of experimental diabetic animal models, genetically induced spontaneous diabetes models (BKS-DB mice)and experimentally induced non-spontaneous diabetes models (STZ-induced diabetic C57BL/6 mice).Method: To detect cell surface antigens of culture expanded MSCs with flow cytometry and multipotential ability with differentiation experiment;To establish one of the diabetes mouse models with STZ intraperitoneal injection and to chose the C57BLKS/Nju spontaneous mutation diabetic mouse (BKS-DB mice) as the other;To establish two 6-mm full-thickness excision skin wounds on back of both diabetic models;To set four groups: STZ-PBS group vs STZ-MSCs group;BKS-PBS group vs BKS-MSCs group.To observe wound healing when MSCs cells were injected intradermally around the wound at four injection sites.Results: full-thickness excisional wound model was successfully established on STZ-induced diabetic C57BL/6 mice and BKS-DB mice;significantly promoted wound closure was found in both kinds of diabetic mice that with intradermal injection of MSCs cells.Conclusion: MSCs enhanced wound healing of different diabetic model, but with different efficiency, which indicated that different Wound microenvironments of the two model exist.