【摘 要】
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Cell sorting based method is widely used to screen clusters of cells during a complete biological process.We applied this technique to obtain populations of human erythroblasts at distinct development
【机 构】
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CAS Key Laboratory of Genome Sciences and Information,Beijing Institute of Genomics,Chinese Academy
【出 处】
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2015 Genomics Frontiers Symposium(2015基因组学前沿研讨会)
论文部分内容阅读
Cell sorting based method is widely used to screen clusters of cells during a complete biological process.We applied this technique to obtain populations of human erythroblasts at distinct developmental stages to take a further insight of erythroid differentiation.Taking advantage of next-generation sequencing technology, integrative analyses of mRNAs, micro RNAs (miRNAs),and long non coding RNAs (lncRNAs) were performed to discover potential regulation among them.Bioinformatics analysis revealed that genes highly expressed in hematopoietic stem cells (HSCs)tended to be down-regulated during erythropoiesis.The most obvious regulation occurred during the initial stage of erythropoiesis according to the expression data of mRNA, miRNA, and lncRNA, and dominated by down-regulation.After that, three kinds of RNAs presented an upward trend.The similar expression pattern of mRNAs, miRNAs, and lncRNAs during erythroid differentiation suggests a potential correlated regulation mechanism of three kinds of RNAs on erythropoiesis.Approximately 60% of differentially-expressed genes were continuously regulated by miRNAs during erythrpoiesis.Differentially-expressed genes regulated by miRNAs during the initial stage of differentiation were associated with macrophage differentiation, myeloid cell differentiation,myeloid leukocyte differentiation, hemopoiesis, and hemopoietic or lymphoid organ development.Ongoing inhibition of Notch signaling pathway during the whole differentiation procedure, which was closely regulated by several miRNAs, was observed and considered to be critical to erythroid differentiation.This study provides a significant resource for studies of erythropoiesis, allowing a deeper understanding of potential regulation between noncoding RNAs and coding genes in normal erythroid development as well as erythroid disorders.
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