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MicroRNAs(miRNAs)play important regulatory roles by targeting mRNAs for cleavage or translational repression.Identification of miRNAtargetsis essential for our better understanding the roles of miRNAs.miRNA targets have not been well characterized in mulberry(Morus alba).In order to anatomize miRNA guided gene regulation under drought stress,transcriptome-wide high throughput degradome sequencing was used in this study to directly detect drought stress responsive miRNA targets in mulberry.Drought library(DL)and contrast library(CL)were constructed to capture the cleaved mRNAs for sequencing.