Coding regions of trnL and matK and intergenic spacer (IGS) region of trnT-L in chloroplast were sequenced to analyze phylogenetic relationship of the genus Tulipa,including 27 cultivars,and 34 wild species,by using Amana edulis as an outgroup.12 variable sites out of 421 base pairs (bps) in the trnL,19variable sites out of 834 bps in the matK,and 62 variable sites out of 733 bps in the trnT-L are detected,indicating that the trnL has evolved more slowly and less informative for the classification.