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目的探讨食管下端括约肌肌间神经丛氮能神经元的生后发育规律。方法分别取生后第1d、7d、14d、21d、30d、成年大鼠的食管下端括约肌及食管体,应用铺片技术与NADPH-d酶组织化学法,定性、定量研究食管下端括约肌与食管体的肌间神经丛内氮能神经元的发育变化。结果随着日龄的增加,食管下端括约肌肌间神经丛内氮能神经元成熟度逐渐增加,神经元密度逐渐下降,从生后第1d的(293.7±47.8)个/mm2,逐渐下降为生后第30d的(25.9±9.9)个/mm2,而食管体的神经元密度明显低于相应组别的食管下端括约肌(P<0.05);神经元大小从生后第1d的(129.8±45.9)μm2增加到生后第30d的(428.3±122.1)μm2,基本达到成年组水平,各组间差异有显著性意义(P<0.01)。结论大鼠食管下端括约肌肌间神经丛内的氮能神经元在生后继续发育,以哺乳期变化为著,至生后30d时基本成熟;与食管体比较,食管下端括约肌氮能神经元更为密集。
Objective To investigate the postnatal development of the myenteric plexus neurons in the lower esophageal sphincter. Methods The esophageal lower esophageal and esophageal bodies of adult rats on day 1, day 7, day 14, day 21, day 30, respectively, were dissected. Using the technique of tissue engineering and NADPH-d enzyme histochemistry, the lower esophageal sphincter and esophageal The development of Nerve neurons in the myenteric plexus. Results With the increase of age, the maturation of NES in myenteric plexus of esophageal lower esophageal gradually increased, and the density of neurons decreased gradually from (293.7 ± 47.8) / mm2 on day 1 postnatal to (25.9 ± 9.9) / mm2 on the 30th day after transplantation, while the neuronal density in the esophagus was significantly lower than that in the corresponding lower esophageal sphincter (P <0.05) μm2 increased to (428.3 ± 122.1) μm2 on the 30th day after birth, reaching the level of the adult group basically. There was significant difference among the groups (P <0.01). Conclusions Nitrogen-able neurons in the myenteric plexus of the lower esophageal sphincter continue to develop after birth, with a change of lactation, and basically mature 30 days after birth. Compared with the esophagus, the N-type neurons of the lower esophageal sphincter Intensive.